Cat. No.
MABL-3548
Application
IP, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
EbolaVirus (Zaire, IvoryCoast, Reston, Sudan)
Clone No.
KZ51
From
Recombinant Antibody
Specificity
This antibody binds to the nucleoprotein of four Ebola virus strains and has a specificity found in convalescent patients. No cross-reactivity with other proteins (e.g. GP) was detected by radioimmunoprecipitation.
Alternative Names
NP; Ebola NP; EBOV NP
UniProt
Immunogen
This antibody was derived from a human survivor of the 1995 Kikwit outbreak of Ebola.
Application Notes
This antibody is broadly cross-reacting between different Ebola strains and competes with antibodies naturally occuring in covalescent patients. By IF, this antibody has higher reactivity towards fixed virus than to live virus - though both may be detected. It has also been used as a positive control in the development of anti-Ebola polyclonal goat antibodies (Boisen et al. 2015).
Antibody First Published
Maruyama et al. Ebola virus can be effectively neutralized by antibody produced in natural human infection. J Virol. 1999 Jul; 73(7): 6024–6030. PMID:10364354
Note on publication
Describes the generation and functional characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
