Cat. No.
MABL-3549
Application
neutralize, therapeutic, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus2
Clone No.
CV1
From
Recombinant Antibody
Specificity
This antibody binds the ectodomain encoded by amino acid residues 1-1208 of the SARS CoV 2 spike protein.
Alternative Names
S-protein; SARS-CoV2; SARS Coronavirus 2; SARS-CoV-2; SARS CoV 2; 2019-nCoV; Severe acute respiratory syndrome coronavirus 2; Spike protein; S protein; SARS-CoV 2 S protein; S glycoprotein; E2; Peplomer protein; SARS-CoV2
UniProt
P0DTC2
Immunogen
The original antibody was generated by isolating B-cells from the peripheral blood mononuclear cells (PBMCs) of SARS CoV2 infected patients, after the serum neutralizing activity was confirmed.
Application Notes
This antibody is recommended for diagnosis and/or treatment of SARS CoV 2 or 2019- nCoV. CV1 binds an epitope outside the SARS CoV 2 RBD was found to be weakly neutralizing (IC50=15µg/ml). ELISA was used to confirm the binding of SARS CoV 2 positive serum samples to the entire ectodomain and the receptor binding domain of the spike protein. Specific binding of the mAb to SARS CoV 2 to ectodomain was confirmed with biolayer interferometry. Phycoerythrin labelled mAb was used to stain 293E cells transfected with wild type SARS CoV 2 and were analysed by flow cytometry (Seydoux et al., 2020).
Antibody First Published
Seydoux et al. Characterization of neutralizing antibodies from a SARS-CoV- 2 infected individual PMID:32511342
Note on publication
Describes the generation and characterization of this antibody from PBMCs of infected patients.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
