Cat. No.
MABL-223
Application
dot blot, IB, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
3-6C1
From
Recombinant Antibody
Specificity
This antibody is specific for the sodium-dependent noradrenaline transporter. This protein is an amine transporter that terminates the action of noradrenaline by its high-affinity sodium-dependent reuptake into presynaptic terminals.
Alternative Names
SLC6A2; Norepinephrine transporter; NET; Solute carrier family 6 member 2; Norepinephrine transporter; NET; Solute carrier family 6 member 2
UniProt
P23975
Immunogen
This antigen was raised by immunizing a BALB/c mouse with the sodium-dependent noradrenaline transporter antigen.
Application Notes
This antibody was used for immunoblotting PANC-1 cells mock-infected or infected with GLV-1h68 or GLV-1h99. This virus expresses the human norepinephrine transporter. This antibody was also used for immunofluorescence on PANC-1 cells mock-infected or infected with GLV-1h68 or GLV-1h99 (viruses that express the human norepinephrine transporter) (Chen et al., 2009; PMID: 19287510). This antibody was used for immunoblotting to assess the expression of the norepinephrine transporter. The samples used were entire cells and plasma membranes from Neuron-like human neuroblastoma SK-N-SH cells (Park et al., 2013; PMID: 23485735). This antibody was used in a Western blot assay to assess the expression of norepinephrine transporter protein in CPAE and SK-N-SH cells (Lee et al., 2005; pmid:16513613). Both a dot blot and immunohistochemistry were performed with this antibody on the green monkey kidney cell line COS-7, the human glioma cell line U373, and the human kidney cell line HEK293. This was done to assess the amount of human norepinephrine transporter expression in these lines (Buursma et al., 2005; PMID: 16330572).
Antibody First Published
Lee et al. Characteristics and regulation of 123I-MIBG transport in cultured pulmonary endothelial cells J Nucl Med. 2006 Mar;47(3):437-42. PMID:16513613
Note on publication
The mechanisms that mediate 123I-MIBG transport in pulmonary endothelial cells were characterized, and the effects of stimuli associated with pulmonary dysfunction were investigated.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
