Cat. No.
MABL-224
Application
WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
FH1D12
From
Recombinant Antibody
Specificity
This antibody is specific for TrkB.T1 peptide Ac-C(dPEG4)FVLFHKIPLDG. TrkB.T1 is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.
Alternative Names
BDNF/NT-3 growth factors receptor; NTRK2; GP145-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B
UniProt
Q16620
Immunogen
This antibody was raised by immunizing male and female 20-week-old C57BL6 mice that were characterized as genetic null for TrkB.T1 with TrkB.T1 peptide Ac-C(dPEG4)FVLFHKIPLDG-OH maleimide coupled to KLH carrier protein.
Application Notes
To validate this antibody, immunohistochemistry was preformed on multi-tissue block containing human brain, mouse brain, TrkB.T1−/− mouse brain, a cell pellet over-expressing TrkB.T1 and a cell pellet without TrkB.T1 were stained using the scFv version of this antibody. Furthermore, the specificity of this antibody was proven using western blot with the mouse version of this clone. Finally, flow cytometry confirmed the specificity of the mouse version of this antibody (Patwell et al, 2020; pmid:32532995).
Antibody First Published
Patwell et al. A kinase-deficient NTRK2 splice variant predominates in glioma and amplifies several oncogenic signaling pathways Nat Commun. 2020 Jun 12;11(1):2977.
PMID:32532995
Note on publication
A role for a truncated NTRK2 splice variant, TrkB.T1, in human glioma was shown.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
