Cat. No.
MABL-3550
Application
neutralize, therapeutic, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus2
Clone No.
CV30
From
Recombinant Antibody
Specificity
This antibody recognizes and binds the receptor binding domain (RBD) encoded by residues 319-591 of the SARS CoV 2 spike protein. CV30 binds the SARS CoV 2 RBD with a high affinity of 3.6nM and completely blocks the interaction between the RBD and ACE2 domain.
Alternative Names
Receptor Binding Domain; SARS-CoV2; SARS Coronavirus 2; SARS-CoV-2; SARS CoV 2; 2019-nCoV; SARS Coronavirus 2 receptor binding domain; SARS CoV2 RBD; Severe acute respiratory syndrome coronavirus 2; Spike protein; S protein; SARS-CoV 2 S protein; S glycoprotein; E2; Peplomer protein
UniProt
P0DTC2
Immunogen
The original antibody was generated by isolating B-cells from the peripheral blood mononuclear cells (PBMCs) of SARS CoV2 infected patients, after the serum neutralizing activity was confirmed.
Application Notes
This antibody is a potent 2019-nCoV neutralizing antibody and highly recommended for diagnosis and/or treatment of SARS CoV 2 or 2019-nCoV. CV30 binds SARS CoV 2 RBD with a very high affinity and achieves 100% neutralization (IC50=0.03µg/ml). ELISA was used to confirm the binding of SARS CoV 2 positive serum samples to the entire ectodomain and the receptor binding domain of the spike protein. Specific binding of the mAb to SARS CoV 2 to receptor binding domain was confirmed with biolayer interferometry. Phycoerythrin labelled mAb was used to stain 293E cells transfected with wild type SARS CoV 2 and were analysed by flow cytometry (Seydoux et al., 2020).
Antibody First Published
Seydoux et al. Characterization of neutralizing antibodies from a SARS-CoV- 2 infected individual PMID:32511342
Note on publication
Describes the generation and characterization of this antibody from PBMCs of infected patients.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
