Cat. No.
MABL-212
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
K19/36R
From
Recombinant Antibody
Specificity
This antibody is specific for Kv1.6. Kv1.6 is a voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient.
Alternative Names
Potassium voltage-gated channel subfamily A member 6; Kcna6; RCK2; Voltage-gated potassium channel subunit Kv1.6; Voltage-gated potassium channel subunit Kv2; K19/36
UniProt
P17659
Immunogen
This antibody was raised by immunizing BALB/c mice with a synthetic peptide 507-525 of rat Kv1.6.
Application Notes
COS-1 cells were used for immunofluorescence with this antibody (Bekele-Arcuri et al, 1996; PMID:8938716). Immunohistochemistry was preformed on rat brains using this antibody (Bekele- Arcuri et al, 1996; PMID:8938716). GST fusion proteins were used for an ELISA with this antibody (Bekele- Arcuri et al, 1996; PMID:8938716). Immunofluorescence was preformed using this antibody on mouse brain cell lysates (Gazula et al, 2010; PMID:20575068). Western blots of COS cell lysates transfected with rat Kv1.6 were preformed (Gazula et al, 2010; PMID:20575068). Brain cell lysates from 6-month-old C57BL/6 mice were immunoblotted with this antibody (Willis et al, 2018; PMID:29564531). Slice cultures from postnatal day 10–11 Sprague-Dawley rats were used for immunohistochemistry with this antibody (He et al, 2012; PMID:22218089). Both tissue from morton’s neuroma patients and sciatic nerve and DRG from rats was immunoblotted with this antibody (Calvo et al, 2016; PMID:27033551). immunohistochemistry was preformed on the sciatic nerve of rats using this antibody (Calvo et al, 2016; PMID:27033551).
Antibody First Published
Bekele-Arcuri et al Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides Neuropharmacology.
1996;35(7):851-65. PMID:8938716
Note on publication
Describes the generation of the anti Kv1.5 antibody
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
