Cat. No.
MABL-211
Application
functional assays, IP, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
K7/45R
From
Recombinant Antibody
Specificity
This antibody is specific for Kv1.5. This antibody recognized a single protein band at 70 kDa on western blot of lysates from Kv1.5-transfected COS cells. No band of equivalent size is observed from rat brain samples and Kv1.5-untransfected COS cells Kv1.5 is a voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient.
Alternative Names
Potassium voltage-gated channel subfamily A member 5; Kcna5; RCK7; RK4; Voltage-gated potassium channel subunit Kv1.5; K7/45
UniProt
P19024
Immunogen
This antibody was raised by immunizing BALB/c mice with a fusion protein of amino acids 542-602 of rat Kv1.5.
Application Notes
COS-1 cells were used for immunofluorescence with this antibody (Bekele-Arcuri et al, 1996; PMID:8938716). Immunohistochemistry was preformed on rat brains using this antibody (Bekele- Arcuri et al, 1996; PMID:8938716). GST fusion proteins were used for an ELISA with this antibody (Bekele- Arcuri et al, 1996; PMID:8938716). Immunofluorescence was preformed on COS-1 cells using this antibody (Murakoshi and Trimmer, 1999; PMID:10024359). Proteins from the atrial myocardium and rat brain tissue was immunoprecipitated using this antibody (Godreau et al, 2002; PMID:12445884). proximity ligation (PLA) assay was preformed with this antibody to detect Kv protein-protein interactions (Kilfoil et al, 2019; PMID:31639389). Immunohistochemistry was preformed with this antibody on 50-um-thick sections of mouse brain (Yamada and Jinno, 2013; PMID:22987820). Splenic DCs from Cd11c-Cre-GFP mice were immunoblotted with this antibody (Dong et al, 2019; PMID:31028120).
Antibody First Published
in: Bekele-Arcuri et al Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides Neuropharmacology.
1996;35(7):851-65. PMID:8938716
Note on publication
Describes the generation of the anti Kv1.5 antibody
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
