Cat. No.
MABL-214
Application
WB, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
L5/1R
From
Recombinant Antibody
Specificity
This antibody is specific for SCG10. SCG10 is a regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone.
Alternative Names
Stathmin-2; Stmn2; Superior cervical ganglion-10 protein; Protein SCG10; L5/1
UniProt
P21818
Immunogen
This antibody was raised against a synthetic peptide of amino acids 155-179 of rat SCG10.
Application Notes
Western blot was preformed on lysed rat neurons using this antibody (Tortosa et al, 2017; PMID:28521134). Immunostaining was preforemed on hippocampal and cortical neurons from rats using this antibody (Tortosa et al, 2017; PMID:28521134). Immunofluorescence was preformed on rat neurons. These neurons were either transfected at 6 DIV using Lipofectamine 2000 or untransfected (Levy et al, 2011; PMID:21471001).
Antibody First Published
Levy et al. Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases Mol Biol Cell. 2011 Jun 1;22(11):1930-42. PMID:21471001
Note on publication
it was shown that palmitoylation of stathmins 2 and 3 likely occurs at the Golgi and is crucial for their specific subcellular localization and trafficking.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
