Cat. No.
MABL-215
Application
IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N3/26R
From
Recombinant Antibody
Specificity
This antibody is specific for Slo2.2. Slo2.2 is an outwardly rectifying potassium channel subunit that may coassemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro).
Alternative Names
Potassium channel subfamily T member 1; Kcnt1; Sequence like a calcium-activated potassium channel subunit; N3/26
UniProt
Immunogen
This antibody was raised against a fusion protein of amino acids 1168-1237 of rat Slo2.2.
Application Notes
Mouse membrane proteins from whole brain, cerebellum, cortex and spinal cord were immunoprecipitated using this antibody (Martinez-Espinosa et al, 2015; PMID:26559620). Immunoblotting was preformed on mouse membrane proteins from whole brain, cerebellum, cortex and spinal cord using this antibody (Martinez-Espinosa et al, 2015; PMID:26559620). Membrane vesicles from mouse fore- and midbrain were immunoprecipitated using this antibody (Rizzi et al, 2015; PMID:29124216). This antibody was used for immunoblotting on membrane vesicles from mouse fore- and midbrain (Rizzi et al, 2015; PMID:29124216). immunofluorescence was preformed on membrane vesicles from mouse fore- and midbrain using this antibody (Rizzi et al, 2015; PMID:29124216). Untransfected HEK cell lysates or HEK cell lysates transfected with either pcDNA3-Slo2.1 or pcDNA3-Slo2.2 or 20 lg of purified synaptic plasma membrane vesicles were immunoblotted with this antibody (Rizzi et al, 2016; PMID:26587966). C57BL/6J mouse brain sections were used for immunohistochemistry using this antibody (Rizzi et al, 2016; PMID:26587966). The expression of Slack and Slick in rats was assessed for cultured VANs, vestibular epithelium sections and for vestibular ganglion sections. This was done using immunohistochemistry with this antibody (Cervantes et al, 2013; PMID:23466807).
Antibody First Published
Zhang et al. Regulation of Neuronal Excitability by Interaction of Fragile X Mental Retardation Protein with Slack Potassium Channels J Neurosci. 2012 Oct 31;32(44):15318-27.PMID:23115170
Note on publication
Aplysia bag cell (BC) neurons, which regulate reproductive behaviors, were used to examine the effects of Slack and FMRP on excitability.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
