Cat. No.
MABL-216
Application
WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
L61/14R
From
Recombinant Antibody
Specificity
This antibody is specific for Kv2.1. Kv2.1 is a voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain, but also in the pancreas and cardiovascular system. Contributes to the regulation of the action potential (AP) repolarization, duration and frequency of repetitive AP firing in neurons, muscle cells and endocrine cells and plays a role in homeostatic attenuation of electrical excitability throughout the brain.
Alternative Names
Potassium voltage-gated channel subfamily B member 1; Kcnb1; Delayed rectifier potassium channel 1; DRK1; Voltage-gated potassium channel subunit Kv2.1
UniProt
Immunogen
This antibody was raised by immunizing a mouse with the pS603 phosphopeptide.
Application Notes
mmunoblotting was preformed on Kv2.1-KO and AMIGO-KO mouse brain samples (Mandikian et al, 2014; PMID:24962901). immunohistochemistry against brain samples from wild-type mice subjected to CO2 inhalation was preformed with this antibody (Mandikian et al, 2014; PMID:24962901). This antibody was validated with ELISA on rat Kv2.1 amino acids 595–616 (Mandikian et al, 2014; PMID:24962901). COS-1 and CHO cells expressing Kv channels were used for immunofluorescence with this antibody (Cobb et al, 2015; PMID:26442584). COS-1 and CHO cell lysates transfected with transfected with rat Kv2.1, Kv1.4, or Kv2.1 were immunoblotted with this antibody (Cobb et al, 2015; PMID:26442584).
Antibody First Published
Mandikian et al. Cell type-specific spatial and functional coupling between mammalian brain Kv2.1 K+ channels and ryanodine receptors J Comp Neurol. 2014 Oct 15;522(15):3555- 74. PMID:24962901
Note on publication
It was revealed that striatal MSNs are distinct in their expression of clustered Kv2.1 at plasma membrane sites juxtaposed to intracellular RyRs, as well as in Kv2.1 phosphorylation state.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
