Cat. No.
MABL-218
Application
ICC, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MN423
From
Recombinant Antibody
Specificity
This antibody is specific for the pronase-resistant core PHF and the 12 kDa tau fragment. It does not recognize normal full-length tau. It is also reported that this antibody specifically recognizes tau truncated at Glu391 on the C terminus side. The addition or removal of a single residue at the C-terminus abolishes immunoreactivity.
Alternative Names
MAPT; TAU; PHF-tau; MAPTL; MTBT1; TAU; PHF; Tau proteins; Alzheimer paired helical filaments; Paired helical filament-tau; Microtubule-associated protein tau; Neurofibrillary tangle protein; mAb 423; NOAL 6.423; NOAL 6/66.423.2
UniProt
P10636
Immunogen
The original antibody was generated by hyper-immunizing C3H mice with tangle-enriched fragments of the Alzheimer paired helical filaments core.
Application Notes
The original format of this antibody was characterized in detail (Novak et al., 1989; PMID: 2602435) and has been extensively utilized in Alzheimer's disease research: It played a crucial role in the structural characterization of the Alzheimer paired helical filament (PHF) core (Wischik et al., 1988; PMID: 2455299), and was employed for extracting two peptide fragments (9.5 and 12 kDa) of the PFH pronase-resistant core through immunoblotting (Wischik et al. 1988; PMID: 3132715). Additionally, it was used to study the folding of the Alzheimer’s core PHF subunit through competitive ELISA and western blotting (Skrabana et al., 2004; PMID: 15196943). It has been instrumental in the molecular investigation of neurofibrillary degeneration in Alzheimer's disease (Bondareff et al., 1994; PMID: 7509849). Epitope mapping revealed that it requires a glycine at position -3 with respect to the C terminus (Khuebachova et al., 2002; PMID: 11983234). In the realm of tau protein studies related to Alzheimer's disease, it has been employed for immunohistochemistry on brain tissue samples (Mondragón-Rodríguez et al., 2014; PMID: 24033439). Double and triple-labeled immunofluorescence was conducted using this antibody on brain tissue from Alzheimer's disease patients (Mondragón-Rodríguez et al., 2014; PMID: 24033439) (García- Sierra et al., 2003; PMID: 12719624). This antibody was used to immunoblot purified candidate tau analogs (Novak et al., 1993; PMID: 7679073). Furthermore, it was employed in immunohistochemical staining of brain tissue from patients with Alzheimer's disease, NCI, PSP, or CBD (Berry et al., 2004; PMID: 15475684). Western blot analysis on brain tissue from Alzheimer's patients was performed (Fitzpatrick et al., 2017; PMID: 28678775). This antibody was also used for a competitive ELISA on the Alzheimer's disease expression cDNA library made from the hippocampus of a 70-year-old patient who died from Alzheimer’s disease (Khuebachova et al., 2002; PMID: 11983234).
Antibody First Published
Wischik et al. Isolation of a fragment of tau derived from the core of the paired helical filament of Alzheimer disease. Proc Natl Acad Sci U S A. (1988); 85(12):4506-10.
PMID:3132715
Note on publication
The original publication describes the generation and use of this antibody for the extraction of two peptide fragments of Alzheimer paired helical filaments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.