Cat. No.
MABL-219
Application
IP, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
21F12
From
Recombinant Antibody
Specificity
This antibody recognizes amino acids 33-42 of amyloid beta.
Alternative Names
Abeta; A-beta; Aβ; Aβ42; Alzheimer disease amyloid protein; S-APP-beta
UniProt
P05067
Immunogen
The original antibody was generated by immunizing A/J mice with a synthetic peptide (NH2- CYS-NH-CH2-(CH2)5-CO-GLMVGGVVIA-COOH) as an immunogen.
Application Notes
This antibody has very high specificity for Aβ1–42 over Aβ1–40. This antibody is capable of immunoprecipitating Aβ peptides ending at aa position 42 and also p3 peptides (PMID: 8917563). This antibody is also recomended for detection of amyloid beta using sandwich ELISA (PMID: 9037091, 19204155). This antibody was also used in the quantification of the proportion of full-length Aβ using western blotting (PMID: 12787077). This antibody has also been used for the identification of Aβ42 plaques on paraffin frontal sections(PMID: 15215183, 19660650). This antibody is unable to bind plaques in unfixed brain sections. This antibody is also not capable of clearing plaque after in vivo administration (PMID: 10932230).
Antibody First Published
Citron et al. Evidence that the 42- and 40-amino acid forms of amyloid beta protein are generated from the beta-amyloid precursor protein by different protease activities. Proc Natl Acad Sci U S A. (1996); 93(23):13170-5. PMID:8917563
Note on publication
Describes the generation of different amino acid forms of amyloid beta by different protease activities.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
