Cat. No.
MABL-220
Application
IP, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
6G1
From
Recombinant Antibody
Specificity
This antibody recognizes a conformational epitope on the human iC3b.
Alternative Names
inactivated C3b; Complement C3; C3; Complement C3 beta chain
UniProt
P01024
Immunogen
The original antibody was generated by immunizing mice intraperitoneally with human iC3b protein mixed with RIBI adjuvant.
Application Notes
This antibody binds iC3b with a dissociation constant of 43.3 nM. This antibody can be used in combination with 5D2 to detect iC3b in a sanwich ELISA. This antibody was used to identify iC3b using immunoprecipitation. This antbody was also used for the immunohistochemical analysis of Alzheimer's disease brain and was successful in labelling plaques with various morphologies in the grey matter at a 0.625 μg/mL dilution. This antibody can successfully stain human bruch's membrane/choroid tissues samples from patients having age related macular degeneration (AMD). It was able to identify drusen associated with bruch's membrane in stage 3 and 4 AMD tissues (US20140127225).
Antibody First Published
US20140127225
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
