Cat. No.
MABL-227
Application
antagonist, neutralizing, SPR, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
6A3
From
Recombinant Antibody
Specificity
This antibody is specific for CD127.
Alternative Names
IL7R; IL7Rα; IL-7Rα; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R-alpha; IL-7RA; CDw127
UniProt
P16871
Immunogen
The original antibody was generated by immunizing Balb/c mice with dimeric recombinant human CD127 extracellular domain.
Application Notes
ELISA was performed on CD127 using this antibody (WO2010017468). This antibody was tested for inhibition of IL-7 induced IFN-γ and IL-17 in a TH17 expansion assay (WO2010017468). Flow cytometry was performed on Mock transfected CHO or CHO-CD127 cells using this antibody (WO2010017468). Surface plasmon resonance was performed with this antibody on CD127 to test the binding affinity (WO2010017468). Immunohistochemistry was performed on the spinal cord of a mouse using this antibody (WO2010017468). A neutralizing assay was performed on CD127 protein using this antibody (WO2010017468). Western blot was performed on protein extract using this antibody (WO2010017468).
Antibody First Published
Leung et al. Treatment of autoimmune and inflammatory disease. WO2010017468 PMID:
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
