Cat. No.
MABL-228
Application
WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
TOC1
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 209-224 of the Tau Oligomeric complex.
Alternative Names
TOC-1;
UniProt
Immunogen
The original antibody was generated by immunizing a mouse with electro eluted cross-linked Tau dimers.
Application Notes
Western blot was performed on recombinant Tau and human brain homogenate using this antibody (US8697076). This antibody was used for immunogold labeling on aggregated tau samples (US8697076) (Ward et al, 2013; pmid:23979027). Tissue from the entorhinal cortex and the hippocampus of Alzheimer’s patients was used for immunohistochemistry using this antibody (US8697076). Tissue from the entorhinal cortex and the hippocampus of Alzheimer’s patients was used for immunofluorescence with this antibody (US8697076). Western blot was performed on different deletion mutants of the Tau protein using this antibody (Ward et al, 2013; pmid:23979027). Immunohistochemistry was performed on tissue sections of severe AD, CBD, and PSP cases from the entorhinal cortex using this antibody (Ward et al, 2013; pmid:23979027). M1C cells were used for immunocytochemistry using this antibody (Shirafuji et al, 2018; pmid:29562600). Proteins from M1C cells were blotted with this antibody (Shirafuji et al, 2018; pmid:29562600). ELISA was performed on S1 fractions from a 9 month old murine PS19 cortex using this antibody (Apicco et al, 2017; pmid:29273772).
Antibody First Published
Ward et al. TOC1: characterization of a selective oligomeric tau antibody. J Alzheimers Dis. 2013;37(3):593-602. PMID:2.40E+07
Note on publication
The TOC1 antibody is charatarized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
