Cat. No.
MABL-229
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Bovine, Rat, Human, Mouse
Clone No.
3F7-B11-B5
From
Recombinant Antibody
Specificity
The antibody recognizes mouse, rat and bovine NOS.
Alternative Names
NOS; Nitric oxide synthase; NOS-3F7-B11-B5
UniProt
E1BKI3
Immunogen
The original antibody was generated by immunizing mice against purified, bovine cerebellum NOS.
Application Notes
The antibody reacted with bovine cerebellum NOS as well as with structurally different NOSs from cultured bovine aortic endothelial cells and cultured RAW 264.7 macrophages in Western blot analysis. The reactivity of the antibody was confirmed by immunohistochemical staining of rat cerebellum, arterial endothelial cells, and cultured stimulated macrophages. The antibody showed immunoreactivity within the respiratory epithelium but not in the bronchial smooth muscle (Rengasamy et al, 1994; pmid:7528982). The antibody was used for immunohistochemistry study to investigate the neuronal nitric oxide synthase immunoreactivity in cats (Papantchev et al, 2006; pmid:17120106). The antibody was used for immunohistochemical study on human colon tissue sample (Amato et al, 2014; pmid:24443715).
Antibody First Published
Rengasamy et al. Immunohistochemical demonstration of a paracrine role of nitric oxide in bronchial function. Am J Physiol. 1994 Dec;267(6 Pt 1):L704-11. PMID:7528982
Note on publication
The article describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
