Cat. No.
MABL-232
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Birds, Clawed frog, Cow, Rat, Human
Clone No.
AA5 (AP18)
From
Recombinant Antibody
Specificity
This antibody is specific to the phosphorylated form of MAP2a, b, c and shows no cross- reactivity with other MAPs, tau or tubulin. This antibody reacts with dendrites and cell bodies of neurons. It is useful in studies of neuron structure in normal and malignant brain tissue and in Alzheimer's. MAP-2 cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged.
Alternative Names
Methionine aminopeptidase 2; MetAP 2; MAP 2; Initiation factor 2- associated 67 kDa glycoprotein; p67; p67eIF2
UniProt
Q3ZC89
Immunogen
The mouse version of this antibody was generated by immunizing mice with taxol-stabalized microtubules from rat brain.
Application Notes
While testing the specificity of the mouse version of this antibody, a western blot on P9 quail and adult rat brain was performed using the mouse version of this antibody. Furthermore, quail cerebellum was immunologically stained using the mouse version of this antibody (Tucker et al, 1988; pmid:3199190). To examine the relationship between changes in tau phosphorylation and loss of MAP2 immunmoreactivity, immunocytochemistry was performed (Ashford et al, 1998; pmid:9786247). While assessing experience-dependent modifications in MAP2 phosphorylation in rat olfactory bulb. a western blot was performed against adult rat olfactory bulb proteins using the mouse version of this antibody.
Furthermore, immunohistochemistry was performed against coronal sections of olfactory bulbs using the mouse version of this antibody (Philpot et al, 1997; pmid:9391014). While studying subependymal giant cell astrocytoma, immunohistochemistry was preformed using the mouse version of this antibody (Lopes et al, 1995; pmid:8928613).
Antibody First Published
Tucker et al. The sequential appearance of low- and high-molecular-weight forms of MAP2 in the developing cerebellum J Neurosci. 1988 Dec;8(12):4503-12. PMID:3199190
Note on publication
It is shown that avian MAP2 also exists as both high- (Mr approximately 260,000) and low-molecular-weight (Mr approximately 65,000) forms whose relative abundance changes during brain maturation, indicating a conserved function for these proteins during vertebrate neuronal morphogenesis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
