Cat. No.
MABL-234
Application
SPR, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
pT231/pS235_1
From
Recombinant Antibody
Specificity
This antibody shows binding affinity towards pT231/pS235 peptide of the human tau protein. The binding affinity and specificity of this antibody relied heavily on the phosphorylation at Thr-231.
Alternative Names
MAPT; TAU; PHF-tau; PHF; MAPTL; MTBT1; Tau proteins; Phospho-Tau (pT231/pS235); Microtubule‐associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau
UniProt
P10636
Immunogen
Application Notes
ELISA was used for the screening of positive clones that bind phosphopeptides. The binding affinity of the IgG antibody towards pT231/pS235 phosphopeptide was found to be Kd = 0.35 nM using surface plasmon resonance. This antibody can recognize the phospho-tau epitopes in full-length human tau. This antibody was also used for the immunohistochemical analysis of the human brain with Alzheimer's disease (PMID: 23148212).
Antibody First Published
Shih et al. An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. J Biol Chem. 2012 Dec 28;287(53):44425-34. PMID:23148212
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
