Cat. No.
MABL-235
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
T53
From
Recombinant Antibody
Specificity
This antibody binds tetrodotoxin (TTX), which is a small molecular weight neurotoxin that occludes voltage-gated sodium channels in nerve and muscle tissue, resulting in respiratory paralysis and death.
Alternative Names
TTX; C11H17N3O8; 4368-28-9; CHEMBL507974; Tetrodoxin; Tarichatoxin; Maculotoxin; Spheroidine; Tetrodontoxin; Fugu poison
UniProt
Immunogen
The original antibody was generated by immunizing mice with chemically made tetrodotoxin complete antigen. Later on a phage display library was constructed from the amplified genes encoding monoclonal antibodies and the clone was isolated after six rounds of biopanning.
Application Notes
This high affinity antibody can be used for the detection of tetrodotoxin and TTX-BSA conjugate in an ELISA.
Antibody First Published
Wang et al. Construction of a single chain variable fragment antibody (scFv) against tetrodotoxin (TTX) and its interaction with TTX. Toxicon. 2014 Jun;83:22-34. PMID:24613546
Note on publication
Describes the generation and structural characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
