Cat. No.
MABL-236
Application
agonist, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
C2
From
Recombinant Antibody
Specificity
The original antibody was generated by grafting of the murine antibody 38B8 CDRs onto human framework regions. The parental murine antibody 38B8 was generated by immunizing mice with human TRKB.
Alternative Names
NTRK2; Trk-B; GP145-TrkB; TrkB tyrosine kinase; Tropomyosin-related kinase; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; Tropomyosin receptor kinase B; tyrosine receptor kinase B
UniProt
Q16620
Immunogen
This antibody binds human TRKB and also cross reacts with mouse and rat TRKB. TRKB is a membrane bound receptor for brain-derived neurotrophic factor (BDNF). Upon neurotrophin binding it phosphorylates itself and members of the MAPK pathway. Signaling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders.
Application Notes
This agonist antibody is derived from the murine parental anti-TRKB antibody 38B8. The binding of this antibody to TRKB blocks binding of BDNF. The original IgG1 version of this antibody has a KD for human TrkB of 2.54 nM, a KD for mouse TrkB of 1.44 nM, and a KD for rat TrkB of 2.29 nM when measured by Biacore at 25° C. The binding affinity of Fab version of this antibody towards human, mouse and rat TRKB was tested using ELISA. This antibody was reported to show greater efficacy in promoting mouse neuron survival than the parental mouse antibody and BDNF. In vivo treatments with this antibody of mice on high fat diet-induced obesity showed changes in body weight. A single intraperitoneal dose of C2 at 2 mg/kg caused a statistically significant body weight reduction compared to vehicle-treated mice as early as day 2 after dosing. The mean maximum weight reduction by C2 antibody was 29.05% by day 14 when the animals were euthanized (US20100196390). This antibody may also find potential application as a therapeutic for Huntington's disease. The original parental mouse was used in one such study as an agonist to promote neuronal cell survival by activating the receptor and down-stream signaling proteins (PMID: 24503862).
Antibody First Published
Yang et al. Agonist Anti-trkb monoclonal antibodies (US20100196390). PMID:
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
