Cat. No.
MABL-237
Application
SPR, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Clostridium botulinum
Clone No.
BotFab 5
From
Recombinant Antibody
Specificity
The antibody is specific for the hemagglutinin portion of the botulinum neurotoxin A and B complexes.
Alternative Names
BOTOX; BoNT/A; Bontoxilysin-A; Botulinum neurotoxin type A1; Botulinum neurotoxin type A; BoNT/B; Bontoxilysin-B; Botulinum neurotoxin type B; botulinum types A and B toxin complexes.
UniProt
P0DPI0; P10844
Immunogen
The original antibody was generated by panning the library against botulinum type B complex.
Application Notes
The Fab was shown by ELISA to be reactive to botulinum types A and B toxin complexes and not reactive to the 150 kDa NT of type A or B that lacked the non-NT proteins found in the complex. (US5932449A) Surface plasmon resonance demonstrated that the Fab displays a slightly tighter equilibrium binding constant towards botulinum toxin B than toxin A. The dissociation rate constants (kd1 and kd2) with botulinum toxin A are 7.1×10^-3 and 2.3×10^-4 s^-1 (ratio 1:2), while with botulinum toxin B the values are 3.7×10^-3 and 5.7×10^-4 s^-1 (ratio 1:3.5). The Fab antibody was used in an indirect flow cytometric immunoassay to detect the presence of pentavalent toxoid A-B-C-D-E and botulinum toxoid A; the antibody detected the presence of botulinum toxoid A. The limit of sensitivity of the Fab fragment for botulinum toxoid is between 5 and 10 ng/ml (PMID: 10945449)
Antibody First Published
Emanuel et al. Detection of botulinum toxin
Note on publication
The patent describes the use of the antibody as an immunosensor for detecting botulinum toxin in food, health care, and military applications.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
