Cat. No.
MABL-238
Application
neutralize, RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
Centruroides noxius (scorpion)
Clone No.
BCF2
From
Recombinant Antibody
Specificity
This antibody binds the mammal-specific Cn2 toxin found in the venom produced by Centruroides noxius Hoffmann (Mexican scorpion). This antibody binds an epitope on the Cn2 toxin that is different from the binding epitope of another anti-Cn2 toxin antibody 6009F.
Alternative Names
scorpion toxin; Beta-mammal toxin Cn2; Toxin 2; Toxin II.9.2.2; scorpion venom
UniProt
P01495
Immunogen
The original antibody was generated by immunizing female BALB/c mice with a conjugate comprising thyroglobulin and a fraction II-9.2 (which contains toxins 2 and 3) of the Centruroides noxius (mexican scorpion) venom in Freund's complete adjuvant.
Application Notes
The binding specificity and characterization of this antibody towards Cn2 toxin of scorpion Centruroicles noxius was tested using ELISA and RIA. This antibody was capable of inhibiting binding of C. noxius toxin 2 to rat brain membranes in vitro and neutralizing the toxin acitivity in vivo in mice (PMID: 1371253). It was reported that the Fab fragment of this antibody was capable of neutralizing both the Cn2 toxin and whole soluble venom of scorpion Centruroides noxius (PMID: 8875771). The scFv version of this atnbidoy did not shoe any neutralization acitivity in vivo due to its less stability (PMID: 15713481).
Antibody First Published
Zamudio et al. Amino acid sequence and immunological characterization with monoclonal antibodies of two toxins from the venom of the scorpion Centruroides noxius Hoffmann. Eur J Biochem. 1992 Feb 15;204(1):281-92. PMID:1371253
Note on publication
Describes the characterization of the toxins from scorpion Centruroides noxius using antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
