Cat. No.
MABL-239
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Centruroides noxius (scorpion)
Clone No.
6009F
From
Recombinant Antibody
Specificity
This antibody binds the mammal-specific Cn2 toxin found in the venom produced by Centruroides noxius Hoffmann (Mexican scorpion). This antibody binds an epitope on the Cn2 toxin that is different from the binding epitope of another anti-Cn2 toxin antibody BCF2. It was also reported that this antibody cross-reacts with other toxins like Cn3, Css2, and Css4 . Cross-reactivity against Css2 was relevant, because this toxin is the major constituent of the venom of Centruroides suffusus (PMID: 21156801).
Alternative Names
scorpion toxin; Beta-mammal toxin Cn2; Toxin 2; Toxin II.9.2.2; scorpion venom
UniProt
P01495
Immunogen
The original antibody was isolated from a human non-immune phage display library by panning the library against Cn2 toxin of scorpion Centruroides noxius.
Application Notes
The binding specificity and characterization of this antibody towards Cn2 toxin of scorpion Centruroicles noxius was tested using ELISA. The epitope mapping of this antibody was performed using competitive ELISA. The scFv version of this antibody binds Cn2 toxin with an affinity of 410pM and capable of neutralizing 2 LD50 of Cn2 toxin when a 1 : 10 molar ratio of toxin-to-antibody fragment was used. It was also able to neutralize 2 LD50 of the whole venom (PMID: 15885107; US7381802). ELISA was also used to determine the cross- reactivity of this antibody towards other toxins like Cn3, Css2, and Css4 (PMID: 21156801).
Antibody First Published
Riaño-Umbarila et al. A strategy for the generation of specific human antibodies by directed evolution and phage display. An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom. FEBS J. 2005 May;272(10):2591-601. PMID:15885107
Note on publication
Describes the generation of this antibody using phage display technique and also describes the affinity maturation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
