Cat. No.
MABL-243
Application
IP, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Bovine, Epstein Barr virus
Clone No.
E2
From
Recombinant Antibody
Specificity
This antibody binds bovine myelin binding protein (MBP) and also shows cross reactivity against Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1). This antibody can recognize both the native and denatured forms of MBP. The MBP isoforms 4 and 14 along with PLP are most abundant protein components of the myelin membrane in the CNS. The smaller isoforms are believed to have an important role in remyelination of denuded axons in multiple sclerosis.
Alternative Names
MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein
UniProt
P02687
Immunogen
The original antibody was isolated from a phage display library of single-chain variable fragments (scFvs), which was constructed from blood lymphocytes of patienst with MS as a potential source of representative MS autoantibodies.
Application Notes
The scFv version of this antibody binds bovine myelin basic protein (MBP) with a binding affinity of 3.9 nM in an ELISA. Both the scFv and the recombinant huamn IgG1 antibody were capable of recognizing MBP and LMP1 proteins in a western blot assay. The recombinant human IgG1 version of this antibody can also be used for the immunoprecipitation of EBV LMP1 protein from a solution (PMID: 21859892).
Antibody First Published
Gabibov et al. Combinatorial antibody library from multiple sclerosis patients reveals antibodies that cross-react with myelin basic protein and EBV antigen. FASEB J. 2011 Dec;25(12):4211-21. PMID:21859892
Note on publication
Describes the isolation of antibodies against myelin basic protein and EBV antigens from a combinatorial library generated from blood lymphocytes of patienst with multiple sclerosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
