Cat. No.
MABL-242
Application
RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
Bovine, Human
Clone No.
845D3
From
Recombinant Antibody
Specificity
This antibody binds human myelin basic protein (MBP), amino acid residues 80-89 (QDENPVVHF). This antibody also cross reacts with bovine MBP comprising amino acids 78-88. The MBP isoforms 4 and 14 along with PLP are most abundant protein components of the myelin membrane in the CNS. The smaller isoforms are believed to have an important role in remyelination of denuded axons in multiple sclerosis.
Alternative Names
MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein; 84- 5D3
UniProt
P02686
Immunogen
The original antibody was generated by hyperimmunization of BALB/c mice with human MBP peptide comprising amino acids from 80-89.
Application Notes
The epitope mapping of this antibody was done using solid phase radioimmunoassay (PMID: 2419434). This antibody can be used in the dection of MBP in a sample using ELISA (PMID: 2466871).
Antibody First Published
Price et al. Multiple epitopes in a dodecapeptide of myelin basic protein determined by monoclonal antibodies. J Immunol. 1986 Apr 1;136(7):2426-31. PMID:2419434
Note on publication
Describes the generation of 4 antibodies against different epitopes of myelin basic protein and their epitope characterization using solid phase radio immunoassay.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
