Anti-Prion protein mAbs [D18](MABL-247)

MABL-247

in vivo, inhibition, IP, SPR, WB, ELISA, FC

Engineer antibody

Bovine, Syrian Hamster, Human, Mouse

D18

Recombinant Antibody

This antibody recognises a discontinuous epitope and binds to residues 132-156 of PrP. Application Notes

PrPC; ASCR; PrP27-30; PrP33-35C; CD230

P04156

PrP deficient (Prnp0/0) mice were immunized with disaggregated PrP 27-30 incorporated into liposomes. The original antibody was generated by panning by panning against dispersed SHaPrP 27-30. Specificity: This antibody recognises a discontinuous epitope and binds to residues 132-156 of PrP.

The specificity of the antibody to recombinant SHaPrP(90-231) and with PrP 27-30 rods was confirmed by ELISA. The epitope mapping of this antibody was done using a paptide-based ELISA. The Fab fragment reacted with cell surface MoPrPc as showed by flow cytometry. The antibody efficiently immunoprecipitated SHaPrPC from transfected CHO cells (Williamson et al. 1998; PMID: 9765500). The ability of of the Fab fragment to inhibit prion propagation in a cultured mouse neuroblastoma cell line infected with PrPSc (ScN2a) was tested. The Fab fragment was found to abolish prion replication and to clear pre-exiting PrPSc, eliminating 50% of PrPSc from the cells within about 24 hours. Therefore, the antibody showed potential as a therapeutic agent. This antibody was used for detection of PrP with the surface of ScN2a cells by flow cytometry (Peretz et al., 2001; PMID: 11507642). The scFv version of the antibody was constructed and the specificity tested by ELISA. The binding affinity of the scFv fragment to PcP was measured by surface plasmon resonance (Kd= 10.1 nM). The scFv antibody gene was delivered to the brain by a recombinant adeno-associated virus, where it delayed the progression of the disease in a mouse model of TSE (Wuertzer et al., 2008; PMID: 18180775). The Fab fragment was conjugated to UltreSmall gold particles and used for immunolabelling for PrPC localization in mouse hippocampus (Mironov et al., 2003; PMID: 12904479). Immunofluorescence was performed on RK13 cells using this antibody (Spagnolli et al., 2021; PMID: 33437023).

Williamson et al. Mapping the Prion Protein Using Recombinant Antibodies. J Virol. 1998 Nov; 72(11): 9413–9418. PMID:9765500

The paper describes the generation of a panel of monoclonal antibodies, which are used as probes for protein conformational study.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.