Cat. No.
MABL-248
Application
immunotherapy, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
F018
From
Recombinant Antibody
Specificity
This antibody reacts with nicotine. Nicotine is a plant alkaloid, found in the tobacco plant and other plants of the nightshade family. It is an addictive central nervous system (CNS) stimulant that causes either ganglionic stimulation in low doses or ganglionic blockage in high doses. It is the main psychoactive ingredient in tobacco products and a major component of cigarettes, which is also is used therapeutically to help with smoking cessation and reduce withdrawal symptoms.
Alternative Names
Nicotin; rac-3-(1-methylpyrrolidin-2-yl)pyridine; CAS: 54-11-5; CHEBI:18723; Nic12
UniProt
Immunogen
Peripheral blood mononuclear cells (PBMC) were isolated from 32 ml of heparinized blood of a Qβ-Nicotine-vaccinated volunteer. Antigen-specific B cells were used for the construction of a Sindbis- based scFv cell surface display library. The original antibody isolated from this antibody after panning against RNase-Nicotine.
Application Notes
The binding characterization of this antibody to nicotine was done using ELISA. The original scFv and human IgG2 version of this antibody binds (S)-(−)-nicotine with an affinity of Kd= 11.5 ±
1.5 nM and Kd= 7.4 ± 1.2 nM respectively. This antibody does not cross react with (−)-cotinine and acetylcholine. It was estimated that IgG2-F018 binds (−)-cotinine about 1000 fold less strongly than (S)-(−)- nicotine. The IgG2 version of this antibody is also capable of recognizing (R)-(+)-nicotine. The IgG2 version of this antibody was used to study the distribution of nicotine in plasma and brain in mice. It was reported that this antibody can reduce the entry of nicotine to the brain by more than 60% (US8344111).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
