Cat. No.
MABL-251
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
10C9
From
Recombinant Antibody
Specificity
This antibody specifically recognizes the ABCDE ring portion of ciguatoxin CTX3C and 51-OH- CTX3C. This antibody does not cross react with other toxins like brevetoxin A (BTX-A), brevetoxin B (BTX-B), okadaic acid (OA), and maitotoxin (MTX). Ciguatoxins are the major causative toxins of ciguatera seafood poisoning (CFP). Ciguatera is a foodborne illness caused by eating reef fish whose flesh is contaminated with ciguatoxins.
Alternative Names
CTX; CTX3C; 51-hydroxy-CTX3C
UniProt
Immunogen
The original antibody was generated by immunizing mice with a synthetic hapten comprising ABCDE ring portion conjugated with KLH.
Application Notes
This antibody can be used to detect ciguatoxins in a sample using ELISA. It was also used in the development of a sandwich ELISA to detect CTX3C at the ppb level. Dissociation constant of this antibody for ciguatoxin CTX3C was Kd = 2.8 nM (PMID: 12812503). This antibody was also used in combination with another antibody 3D11 for effective neutralization of ciguatoxins in vitro and in vivo (PMID: 19254735).
Antibody First Published
Oguri et al. Synthesis-based approach toward direct sandwich immunoassay for ciguatoxin CTX3C. J Am Chem Soc. 2003 Jun 25;125(25):7608-12. PMID:12812503
Note on publication
Monoclonal antibodies against both ends of ciguatoxins were generated for the development of sandwich immunoassay for detection of ciguatoxin CTX3C.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
