Cat. No.
MABL-252
Application
therapeutic, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
5E 6
From
Recombinant Antibody
Specificity
This antibody binds human TNFR2 (TNFRSF1B). TNFR2 is a receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha.
Alternative Names
CD120b; TNFRSF1B; TNFBR; p75; TNF-R2; TNF-RII; TNFR-II; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor type II; p80 TNF-alpha receptor; Etanercept; 10-5E6; 5E6-mHvKv
UniProt
P20333
Immunogen
The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with recombinant human TNFR2 protein.
Application Notes
The binding characterization to human TNFR2 was done using ELISA. This antibody can effectively block the binding of TNFR2 with TNF alpha. The binding affinity of the original IgG antibody for human TNFR2 was determined using surface plasmon resonance and found to be Kd= 9.53 X 10^(-10) M. This antibody was also reported to show tumor inhibitory activity in b-HTNFR2 mice (colon cancer cells) (CN112480259).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
