Cat. No.
MABL-253
Application
crystallization, in vivo, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Cow, Guinea Pig, Monkey, Rat, Human, Mouse
Clone No.
8.18-C5
From
Recombinant Antibody
Specificity
The antibody binds specifically to MOG, recognising a conformational epitope. MOG is a transmembrane glycoprotein expressed on the surface of oligodendrocytes in the central nervous system, that plays an important role in maintaining the structure of the myelin sheath. Monoclonal antibodies that target MOG are involved in the pathogenesis of the demyelinating disorder multiple sclerosis (MS). Animals injected with anti-MOG antibodies have been shown to display symptoms of human MS, and can thereby be used as models to study the development of the disease. The epitope interacting with the antibody is expressed in rat, mouse, guinea pig, cow, monkey, and humans.
Alternative Names
Myelin-oligodendrocyte glycoprotein; 8.18C5; 8-18C5
UniProt
Q63345
Immunogen
The original antibody was generated immunizing mice against Thy-1-depleted rat cerebellar glycoproteins.
Application Notes
This antibody was detected in white matter tracts of the central nervous system by immununohistochemistry. The antibody detected MOG by western blot analysis (Linnington et al., 1984; PMID: 6207204). The specificity of the original format of the antibody and Fab fragment was confirmed by ELISA analysis (Litzenburger et al., 1998; PMID: 9653093 and Menge et al., 2011; PMID: 22093619). This antibody was used for detection of MOG expressed on B cells by flow cytometry (Na et al., 2021; PMID: 34149706). MOG from HeLa cells were immunoprecipitated with the antibody. Immunofluorescence was performed on HeLa cells using this antibody (Boyle et al., 2007; PMID: 17573820). The antibody triggered in vivo expansion of CD4+ T cells in spleen, inguinal, and cervical lymph nodes (Kinzel et al. 2016; PMID:27022743). The crystal structure of the Fab version of this antibody was determined in complex with MOG Igd (Breithaupt et al., 2003; PMID: 12874380).
Antibody First Published
Linnington et al. A novel myelin-associated glycoprotein defined by a mouse monoclonal antibody. J Neuroimmunol. Sep-Oct 1984;6(6):387-96. PMID:6207204
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
