Cat. No.
MABL-3221
Application
crystallization, in vivo, inhibition
Isotype
Engineer antibody
Species Reactivity
Baboon, Human
Clone No.
82D6A3
From
Recombinant Antibody
Specificity
This antibody is specific for human vWF, A3 domain.
Alternative Names
human vWF A3 domain; VWF-A3; vWF
UniProt
P04275
Immunogen
The original antibody was purified from murine ascites by Protein A chromatography.
Application Notes
This antibody was used as an inhibitor to study platelet adhesion to collagen VI and ECM. It was found to inhibit the binding of von Willebrand factor (VWF) to collagen I and III (Hoylaerts et al., 1997; PMID: 9164855). This antibody was found to have a comparable affinity for baboon vWF as for human vWF and inhibited the binding of baboon vWF to collagen with an inhibitory concentration of 50% (IC50) of 3.5 μg/ml. Furthermore, it prevented the binding of VWF to collagen and removed bound VWF from a collagen surface, albeit with a lower efficacy. Moreover, the antithrombotic efficacy of the antibody was evaluated in a modified Folts model in baboons; this antibody was used to inhibit the interaction between collagen and vWF to prevent arterial thrombosis in baboons (Wu et al., 2002; PMID: 11986216). The crystal structure of the antibody's Fab fragment, in complex with the vWF A3 domain, was determined (Staelens et al., 2006; PMID: 16314412).
Antibody First Published
Hoylaerts et al. von Willebrand factor binds to native collagen VI primarily via its A1 domain Biochem J. 1997 May 15;324 ( Pt 1)(Pt 1):185-91. doi: 10.1042/bj3240185 PMID:9164855
Note on publication
The original publication investigates the interaction mechanism between collagen VI and von Willebrand factor (vWF).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
