Cat. No.
MABL-3222
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Vibrio parahaemolyticus
Clone No.
FA7
From
Recombinant Antibody
Specificity
This antibody is specific for the VP1694 protein from Vibrio parahaemolyticus. This proteins is necessary for this bacteria to act as a pathogen. Vibrio parahaemolyticus can cause gastroenteritis in humans.
Alternative Names
Type III export protein YscF
UniProt
Q87P24
Immunogen
This antibody was generated by panning a scFv library from BALB/c mice immunized with purified fusion protein against GST-VP1694 antigen.
Application Notes
An ELISA was performed on associated V. parahaemolyticus proteins (TDH, TLH, VopQ, VP1694), Homologous protein HrpA, and PBS using the scFv version of this antibody. It showed a stronger binding to VP1694 than to other proteins. Furthermore, a western blot was performed on VP1694 using this antibody. The western blot showed the binding of VP1694 by the antibody (Wang et al, 2014; pmid:25320414).
Antibody First Published
Wang et al. Generation and Characterization of a scFv Antibody Against T3SS Needle of Vibrio parahaemolyticus. Indian J Microbiol. 2014 Jun; 54(2): 143–150. PMID:25320414
Note on publication
A phage single-chain fragment variable antibody (scFv) library was constructed, and a specific scFv against VP1694 named scFv-FA7 was screened by phage display panning.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
