Cat. No.
MABL-3223
Application
crystallisation, SPR, WB, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
Nb119
From
Recombinant Antibody
Specificity
Nb119 is able to cross react with both murine and human VSIG4, showing a 250-fold higher affinity with the mouse protein (Wen et al, 2017). Shown crystallographically, Nb119 binds to the variable immunoglobulin domain of both murine and human VSIG4 at the same epitope, contacting beta-strands at the IgV C-terminus. VSIG4 is a dual-functional regulator of T cell immune response and complement receptor. It is a molecular marker of liver disease and arthritis in mice. Recently, it has been found overexpressed on tumour-associated macrophages, and may play a role in non-small cell lung cancer tumourigenesis.
Alternative Names
Z39Ig; V-set and immunoglobulin domain-containing protein 4; Complement receptor of the Ig superfamily; CRIg; Protein Z39Ig; Z39Ig; Vsig4
UniProt
Q9Y279
Immunogen
The extracellular domain of recombinantly-produced mouse VSIG4 was used to immunise an alpaca (Vicugna pacos). Peripheral blood lymphocytes isolated from the immunised alpaca were used to generate a Nanobody phage library, clones from which were selected for binding to the same recombinant mouse VSIG4 extracellular domain.
Application Notes
Nb119 was shown to exhibit an affinity for murine VSIG4 of 0.9 nM by SPR (Zheng et al, 2014) or 3.5 nM by ITC (Wen et al, 2017). It was further shown by flow cytometry that Nb119 recognised mouse VSIG4 expressed on liver macrophages. Nb119 was crystallised in complex with both murine and human VSIG4 (Wen et al, 2017).
Antibody First Published
Zheng et al. Molecular Imaging with Macrophage CRIg-Targeting Nanobodies for Early and Preclinical Diagnosis in a Mouse Model of Rheumatoid Arthritis J Nucl Med. 2014 May;55(5):824-9. PMID:24686780
Note on publication
Describes the generation of the Nb119 Nanobody from a phage library against murine VSIG4. The Nanobody was subsequently radiolabelled and used for live imaging in arthritic mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
