Cat. No.
MABL-3228
Application
neutralization, ELISA
Isotype
Engineer antibody
Species Reactivity
Vesicular stomatitis Indiana virus
Clone No.
M55
From
Recombinant Antibody
Specificity
This antibody reacts with a defined subsite within the major antigenic site of VSV-G. The exact epitope is not defined.
Alternative Names
Glycoprotein G; Glycoprotein; G
UniProt
P0C2X0
Immunogen
The original antibody was generated by immunizing SCID BALB/c mice with vesicular stomatitis virus (VSV).
Application Notes
This stable, recombinant single-chain Fv-Cκ (scFv-Cκ) antibody fragment was generated by protoplast fusion of J558L myeloma cells with Escherichia coli K803 transformed with an appropriate scFv-Cκ expression construct. The aptly named M55 scFv-Cκ expression construct — containing a CDR2 Ser55-to-Arg substitution (T-to-G transition at the third base), found in the VH chain of several hypermutated antibodies (VI22, VI43, and VI53) — was derived from the secondary antibody clone VI24. M55 binds a defined subsite within the major antigenic site of VSV-G. However, the exact binding site in the subunit is not defined. M55 binding to VSV-G was measured using solid-phase ELISA. The VH Ser55-to-Arg substitution in M55, which is not found in its parental clone VI24, increased monovalent binding by about 10-fold compared with M0 (the germline derived scFv-Cκ). Furthermore, crosslinking with HRP labeled mouse anti-Cκ antibodies improved M55 binding 10-fold (Kalinke et al., 2000; PMID: 10963674).
Antibody First Published
Kalinke et al. Virus neutralization by germ-line vs. hypermutated antibodies Proc Natl Acad Sci USA. 2000 Aug 29;97(18):10126-31. doi: 10.1073/pnas.97.18.10126 PMID:10963674
Note on publication
The original publication describes the creation of scFv-Cκs (including M55), examines their structure, and evaluates their binding and neutralizing capabilities against VSV-G.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
