Cat. No.
MABL-3229
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
F8/44/20
From
Recombinant Antibody
Specificity
This antibody recognises human von Willebrand factor (also know as Factor VIII related antigen). It is produced by endothelial cells and megakaryocytes and is involved in the process of hemostasis by engaging in adhesion of platelets to the sites of vascular injury. Mutations in this gene lead to Von Willebrand's disease which causes frequent bleeding.
Alternative Names
von Willebrand factor; von Willebrand antigen 2; von Willebrand antigen II; coagulation factor VIII VWF; factor VIII-related antigen; F8; F8VWF; VWD
UniProt
P04275
Immunogen
This antibody was raised against von Willebrand factor isolated from human plasma.
Application Notes
IHC: F8/44/20 antibody was used in an Immunohistological analysis of human bone marrow trephine biopsies where it stained megakaryocytes, platelets and endothelial cells in frozen samples (Falini et al., 1984). Furthermore, arsanilated version of this antibody stained cryostat sections of human tonsil strongly labelling endothelial cells (Falini et al., 1986).
Antibody First Published
Naiem et al. The value of immunohistological screening in the production of monoclonal antibodies. J Immunol Methods. 1982;50(2):145-60. PMID:6806388
Note on publication
This article describes generation of anti-vWF antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
