Cat. No.
MABL-3233
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
JG7
From
Recombinant Antibody
Specificity
This antibody is specific for VWF propeptide (VWFpp) and not to the yeast used for the expression of the propeptides.
Alternative Names
von Willebrand factor propeptide
UniProt
Immunogen
The original antibody was generated by selecting antibody fragments using phage display technology, where two phage display libraries were amplified and used to screen for binding to von Willebrand factor propeptide (VWFpp) displayed on yeast cells.
Application Notes
This antibody's original format (scFv) was used in a sandwich ELISA to determine the VWF propeptide levels in plasma and showed a higher binding affinity for VWFpp in plasma at especially lower plasma concentrations (Meiring et al., 2018; PMID: 29992156).
Antibody First Published
Meiring et al. The Use of Phage Display and Yeast Based Expression System for the Development of a Von Willebrand Factor Propeptide Assay: Development of a Von Willebrand Factor Propeptide Assay Biomed Res Int. 2018 May 24;2018:6232091. doi: 10.1155/2018/6232091 PMID:29992156
Note on publication
The original publication describes the development of a cost-effective assay for the diagnosis of von Willebrand disease, focusing on the detection of von Willebrand factor propeptide using phage display and yeast-based expression systems.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
