Cat. No.
MABL-3234
Application
WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
34
From
Recombinant Antibody
Specificity
This antibody binds to the T-cell receptor alpha chains.
Alternative Names
TCR variable alpha chain; T-cell receptor variable alpha chain
UniProt
P01848
Immunogen
Purified recombinant soluble TCR from the T-cell hybridoma 85.33, corresponding to V alpha 11
Application Notes
The antibody binds specifically to the recombinant and native variable domain of the α chain which comprises the T-cell receptor (TCR). The TCR is a disulfide-linked heterodimeric protein expressed on the surface of T-cells in complex with CD3 and ζ-chain molecules. The receptor functions in the recognition of MHC molecule and the subsequent activation of T-cells. The monoclonal antibody 34 recognises Vα regions of TCRs expressed in the 85.33 arthritogenic T-cell hybridoma and can be used to analyse TCR V gene expression and for immunotherapy in T cell mediated autoimmunity.
Antibody First Published
Popov S, Hubbard JG, Ward ES. A novel and efficient route for the isolation of antibodies that recognise T cell receptor V alpha(s). Mol Immunol. 1996 Apr;33(6):493-502. PMID:8700165
Note on publication
Describes the isolation of scFv fragments that recognise both the recombinant and native Vα chain expressed on the surface of T-cells, and their characterization.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
