Cat. No.
MABL-3249
Application
WB
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
7B7/3
From
Recombinant Antibody
Specificity
This antibody is specific to human XRCC2 (it does not bind to other RAD51 paralogs). XRCC2 is a paralog of RAD51 which is a homologue of RecA (E. coli). It is engaged in homologous recombination repair (HRR) pathway of double-stranded DNA of chromosomal fragmentations and deletions.
Alternative Names
XRay Repair Cross Complementing 2; XRay Repair Cross Complementing Protein 2; DNA repair protein XRCC2; X-ray repair complementing defective repair in Chinese hamster cells 2; XRCC2 7B7; 7B7
UniProt
O43543
Immunogen
This antibody was raised by immunising mice with the denaturated recombinant human XRCC2 protein which had been previously amplified and purified from E. coli.
Application Notes
WB: XRCC2 7B7/3 (7B7) antibody was used to visualise XRCC2 on Western blots in multiple experiments (e.g. after previous gel filtration of infected pf9 cells; after immunoprecipitation with different antibodies and others) in a study on the RAD51 paralogs' complexes and their role in genetic recombination (Masson et al., 2001).
Antibody First Published
Masson et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genes Dev. 2001 Dec 15;15(24):3296-307. PMID:11751635
Note on publication
This article describes the earliest use of XRCC2 7B7/3 (7B7/3) antibody to detect XRCC2 protein via Western blotting. However, the exact process of generation of this antibody is described in the following paper (where similar antibodies were generated): Masson, J. Y., Stasiak, A. Z., Stasiak, A., Benson, F. E., & West, S. C. (2001). Complex formation by the human RAD51C and XRCC3 recombination repair proteins. Proceedings of the National Academy of Sciences, 98(15), 8440-8446.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
