Cat. No.
MABL-3250
Application
ICC/IF, WB
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
10F1/6
From
Recombinant Antibody
Specificity
This antibody is specific for XRCC3 and does not cross-react with Rad51B, Rad51C, Rad51D, Rad51, or XRCC2 in Western blot analysis. XRCC3 is a protein involved in the homologous recombination repair (HRR) pathway in chromosomal fragmentations and deletions.
Alternative Names
X-Ray Repair Cross Complementing 3; X-ray repair cross-complementing protein 3; DNA repair protein XRCC3; X-ray repair complementing defective repair in Chinese hamster cells 3; CMM6; 10F1
UniProt
O43542
Immunogen
This antibody was raised by immunising mice with the denaturated recombinant human XRCC3-His6 protein which had been purified from 2.4 liters of E. coli FB810 recA− pLysS carrying pXRCC3- 28c.
Application Notes
WB: XRCC3 10F1/6 antibody was used to visualise XRCC3 on Western blots in multiple experiments (e.g. after previous gel filtration of infected pf9 cells; after immunoprecipitation with different antibodies and others) in a study on the RAD51 paralogs' complexes and their role in genetic recombination (Masson et al., 2001). WB: Sage et al. (2010) used this antibody to visualise XRCC3 protein on Western blots from lysates of HCT116 cells in the experiments demonstrating engagement of Rad51 proteins in mtDNA maintenance. ICC/IF: XRCC3 protein was immunostained with XRCC3 10F1/6 antibody in HEK293 cells (some of the cells had been subjected to the ionising radiation) in an experiment investigating the exact role of XRCC3 in homologous recombination (Forget et al., 2004).
Antibody First Published
Masson et al. Complex formation by the human RAD51C and XRCC3 recombination repair proteins. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8440-6. PMID:11459987
Note on publication
This article describes the detailed generation of this anti-XRCC3 antibody as well as successfully applies it in Western analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
