Cat. No.
MABL-3251
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Yersinia pestis
Clone No.
6B5
From
Recombinant Antibody
Specificity
This antibody recognises Y. pestis surface antigen other than F1 (Western blot analysis showed that this antigen is an outer membrane protein of ca. 30 kDa). Y. pestis It is a facultative anaerobe that can infect humans causing plague. 6B5 antibody also exhibits minor cross-reactivity with Y. pseudotuberculosis. It does not cross-react with the following species: Y. enterocolitica, Salmonella Typhimurium, Salmonella Enteritidis, Salmonella Cerro, Salmonella Montevideo, Salmonella Medegridis, C. freundii, L. monocytogenes, L. innocua, E. coli, E. coli O157:H7, E. coli O111:NM, E. coli O26:H11, S. marcescens, V. cholerae, K. pneumoniae, K. oxytoca, A. viridans, H. alvei, S. flexneri, S. sonnei, P. hauseri.
Alternative Names
non-fraction-1 cell membrane; Yersina pestis surface antigen; Y. pestis non-fraction-one cell membrane antigen; F1 deficient Yersinia pestis; Yersinia pestis F1 deficient antigen; fraction 1 antigen; Yersinia pestis; Pasteurella pestis; Bacterium pestis; Bacille de la peste
UniProt
Immunogen
This antibody was raised by immunising Balb/c mice with formalin-killed F1 antigen–deficient Yersinia pestis A1122 cells.
Application Notes
This antibody allows detection of Yersinia pestis in room temperature. Most available immunosassays for Y. pestis are based on detection of F1, the dominant surface antigen expressed by the pathogen when it is grown at 37C. The advantage of detecting Y. pestis at room temperature is that it allows contamination testing in water, juice, milk, food and other environmental samples (Zhao et al., 2012). This antibody is recommended for the detection of Yersinia pestis in various samples as well as for the research and construction of Y. pestis tests and diagnostic tools.
Antibody First Published
Zhao et al. Detection and isolation of Yersinia pestis without fraction 1 antigen by monoclonal antibody in foods and water. J Food Prot. 2012 Sep;75(9):1555-61. doi: 10.4315/0362-028X.JFP-11-514. PMID:22947461
Note on publication
This article describes the generation and characterisation of BpaB#4 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
