Cat. No.
MABL-3861
Application
IP, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
6D5
From
Recombinant Antibody
Specificity
This antibody recognises mouse CD19, which is a transmembrane glycoprotein that regulates B lymphocyte development, activation, and differentiation. This clone 6D5 recognizes the same epitope as the published clone 1D3.
Alternative Names
Leu12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19 T-cell surface antigen Leu-12; CD19; B4
UniProt
P15391
Immunogen
This antibody was raised by immunising rats with mouse CD19-expressing K562 human erythroleukemia cells.
Application Notes
6D5 antibody has been extensively used to identify B cells in various experimental arrangements. It can be efficiently utilised in the localisation of B cells in various tissues. For instance, one group stained murine spleen tissue cryosections to visualise lymphomas in azathioprine-treated mice (Chalastanis et al., 2010). This antibody has also been widely used in flow cytometry. One group utilised in the flow cytometric identification of B cells in a study investigating the relation of microglia depletion and MPTP neurotoxicity (Yang et al., 2018).
Antibody First Published
Krop et al. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan;26(1):238-42. PMID:8566073
Note on publication
This article describes the method of generation of antibodies like 6D5.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
![Anti-Beta-galactosidase [13R4]](/public/static/common/images/not_adv.jpg)
