Cat. No.
MABL-4003
Application
negative control, WB, FC
Isotype
Engineer antibody
Species Reactivity
E. coli
Clone No.
13R4
From
Recombinant Antibody
Specificity
13R4 recognizes wild-type E. coli beta-galactosidase.
Alternative Names
beta-gal; Acid beta galactosidase; Beta galactosidase 1; β-galactosidase; β-gal; EBP; ELNR1; Galactosidase beta 1; GLB1; MPS4B
UniProt
P00722
Immunogen
Beta-galactosidase.
Application Notes
Antibody First Published
Martineau P, Jones P, Winter G. Expression of an antibody fragment at high levels in the bacterial cytoplasm. J Mol Biol. 1998 Jul 3;280(1):117-27. PMID:9653035
Note on publication
Describes the parent scFv13 antibody improved by four rounds of random mutagenesis leading to optimised scFv13R4; scFv13R4 was selected for its capacity to fold correctly in a reducing environment such as the cytoplasm.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
![Anti-Beta-galactosidase [13R4]](/public/static/common/images/not_adv.jpg)
