Cat. No.
MABL-4005
Application
in vivo, negative control
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
4-4-20 (enhanced)
From
Recombinant Antibody
Specificity
This antibody is specific for fluorescein I.
Alternative Names
FL; FlI; FITC; Fluorescein isothiocyanate; fluorescein isothiocyanate, isomer 1; Fluorescyl; Resorcinolphthalein; C.I. 45350; solvent yellow 94; D & C yellow no. 7; Angiofluor; Japan yellow 201; Soap yellow; 3′,6′-dihydroxyspiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with fluorescein I-keyhole limpet hemocyanin (FlI-KLH).
Application Notes
This antibody, a humanized, enhanced scFv of the original mouse IgG2a antibody, was constructed by grafting the CDRs of the original antibody onto the humanized 4D5 anti-HER2 antibody framework, and subsequently enhancing it with respect to folding and stability via randomization in the form of DNA shuffling, as well as oligonucleotides encoding mutations at defined positions (Jung et al., 1999; PMID: 10556036). The original antibody was tested against FlI-BSA. The enhanced antibody fragment was tested against FITC-BSA. This antibody can be used as an isotype control in both in vitro and in vivo applications. This antibody is not conjugated to fluorescein.
Antibody First Published
Jung et al., Selection for improved protein stability by phage display. J Mol Biol. 1999 Nov 19;294(1):163-80. PMID:10556036
Note on publication
The original publication explores the selection of improved protein stability using phage display, generating mutants of the single-chain Fv fragment variant of clone 4-4-20 and comparing selection strategies under different conditions, ultimately yielding a significantly more stable mutant with 20-fold-enhanced binding properties.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
![Anti-Beta-galactosidase [13R4]](/public/static/common/images/not_adv.jpg)
