Cat. No.
MABL-3883
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BrE-3
From
Recombinant Antibody
Specificity
The antibody binds to BEM with a Kd of 37.5 nM.
Alternative Names
BEM
UniProt
P15941
Immunogen
20x10^6 SKBR3 human breast tumour cells were injected into BALB/c mice. The hybridomas generated from the mice spleen cells were then screen for antibodies reactive to c-erbB-2 using immunofluorescence and immunoprecipitation.
Application Notes
Immunofluorescence and immunoprecipitation were used to confirm the specificity of FWP51 for c-erbB-2 (Harwerth et al, 1992). Flow cytometry showed that FWP51 binds the extracellular domain of c-erbB-2 (Harwerth et al, 1992). FWP51 has also been fused to the bacterial toxin, exotoxin A. The resultant fusion protein Fv(FWP51)-ETA selectively inhibited protein synthesis in cells expressing high levels of human c-erbB-2 protein, but not in cells expressing little to no human c-erbB-2 protein.Administration of FWP51 to mice impanted with human erbB-2 transformed NIH3T3 cells led to inhibition of the onset of tumour growth (Harwerth et al, 1993).
Antibody First Published
Harwerth et al, 1992. Monoclonal antibodies against the extracellular domain of the erbB-2 receptor function as partial ligand agonists. J Biol Chem. 1992 Jul 25;267(21):15160-7. PMID:1353079
Note on publication
Describes the original production of the antibody and its characterisation through immunofluorescence and immunoprecipitation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
