Cat. No.
MABL-201
Application
IP, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N83/48R
From
Recombinant Antibody
Specificity
This antibody is specific for GIT-2. GIT-2 is a GTPase-activating protein for the ADP ribosylation factor family.
Alternative Names
ARF GTPase-activating protein GIT2; ARF GAP GIT2; Cool-interacting tyrosine-phosphorylated protein 2; CAT-2; CAT2; G protein-coupled receptor kinase-interactor 2; GRK- interacting protein 2; N83/48
UniProt
Q14161
Immunogen
This antibody was raised against a fusion protein of amino acids 450-759 of human GIT2.
Application Notes
This antibody is recommended for detection and analysis of GIT2 by immunohistochemistry, immunoprecipitation and western blot. For instance, the mouse version of this antibody was used to detect GIT2 by western blot in : extracts of rat brain membranes (RBM) and COS cells transiently transfected with Flag-tagged GIT1 and GIT2 and untagged Kv2.1 plasmids. Furthermore it was used to detect GIT2 by immunohistochemistry in adult rat hippocampus.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
dscribes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
