Cat. No.
MABL-202
Application
WB
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
N69/46R
From
Recombinant Antibody
Specificity
This antibody is specific for the shank3. It shows no cross-reactivity against Shank1 or Shank2. Shank3 is a major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapsee formation, maturation and maintenance.
Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton.
Alternative Names
SH3 and multiple ankyrin repeat domains protein 3; Proline-rich synapsee-associated protein 2; ProSAP2; SPANK-2; N69/46
UniProt
Q9JLU4
Immunogen
This antibody was raised against synthetic peptide of amino acids 840-857 of rat Shank3.
Application Notes
This antibody was used for immunoblotting on hemibrains of wild-type, heterozygous and homozygous Shank3 mice (Bozdagi et al, 2010; PMID:21167025). hemibrains of wild-type, heterozygous and homozygous Shank3 rats were used for immunoblotting with this antibody (Harony- Nicolas et al, 2017; PMID:28139198). Half brains of mice were immunoblotted with this antibody (Kolevzon et al, 2010; PMID:21062623). Western blotting was preformed with this antibody on mouse tissue (Nikoletopoulou et al, 2017; PMID:28683289).
Antibody First Published
Kolevzon et al. Analysis of a purported SHANK3 mutation in a boy with autism: clinical impact of rare variant research in neurodevelopmental disabilities Brain Res. 2011 Mar 22;1380:98-105. PMID:21062623
Note on publication
Our molecular analysis of a child with a purported disruptive mutation in SHANK3 identified by a commercial genetic testing laboratory and we provide evidence that this was not an etiological variant was presented.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
