Cat. No.
MABL-203
Application
ICC, WB, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
N328B/37R
From
Recombinant Antibody
Specificity
This antibody is specific for ZNF423. The antibody does not cross-react with ZNF521. ZNF423 is a transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis.
Alternative Names
Zinc finger protein 423; Olf1/EBF-associated zinc finger protein; hOAZ; Smad- and Olf-interacting zinc finger protein; N328B/37
UniProt
Q2M1K9
Immunogen
This antibody was raised against a fusion protein of amino acids 1-180 of human ZNF423
Application Notes
This antibody is recommended for detection and analysis of ZNF423 by immunocytochemistry, immunohistochemistry or western blot. For instance, the mouse version of this antibody was used to detect ZNF423 by western blot in s crude low-speed pellet fractions from adult rat brain and neonate wild-type (WT) and nur12 (ZNF423 knockout or KO) mouse cerebella. Furthermore it was used to detect ZNF423 by immunocytochemistry in embryonic wild-type (WT) and nur12 (ZNF423 knockout) mouse cerebella.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
Describes the generation of a library of recombinant monoclonal antibodies (R- mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
