Cat. No.
MABL-204
Application
ICC, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Mouse
Clone No.
N295B/66R
From
Recombinant Antibody
Specificity
This antibody was specific for Arl13b. It does not cross-react with Arl13a. Arl13b is a cilium- specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B.
Alternative Names
ADP-ribosylation factor-like protein 13B; ADP-ribosylation factor-like protein 2-like 1; ARL2-like protein 1; N295B/66
UniProt
Q3SXY8
Immunogen
This antibody was raised against a fusion protein of amino acids 208-427 of mouse Arl13b.
Application Notes
Rat E11.5 embryonic spinal cords were used for immunofluorescence with this antibody (Ferent et al, 2019; PMID:31825820). Immunoblotting was preformed on lysated mouse tissue using this antibody (Gigante et al, 2020; PMID:32129762). Arl13bhnn MEFs were immunostained with this antibody (Revenkova et al, 2018; PMID:29592971). Immunofluorescence was preformed on mouse kidney cells using this antibody (Hwang et al, 2019; PMID:30799239). Immunohistochemistry was preformed against mouse embryos using this antibody (Fiore et al, 2020; PMID:32169553). X. tropicalis tissues were immunostained with this antibody (Saurabh et al, 2018; PMID:30377171). Immunocytochemistry was preformed on MEFs with this antibody (Muñoz-Estrada et al, 2019; PMID:31391239). This antibody was used for immunohistochemistry on mouse tissues (Seungshin et al, 2016; PMID:27261005).
Antibody First Published
Seungshin et al. Mutations in Dnaaf1 and Lrrc48 Cause Hydrocephalus, Laterality Defects, and Sinusitis in Mice G3 (Bethesda). 2016 Aug 9;6(8):2479-87. PMID:27261005
Note on publication
Decribes the use of this antibody in an immunohistochemistry experiment.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
