Cat. No.
MABL-205
Application
ICC, WB
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
N129A/6R
From
Recombinant Antibody
Specificity
This antibody is specific for Mad3. Mad3 is an essential component of the mitotic checkpoint. It is required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle.
Alternative Names
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1; N129A/6
UniProt
O60566
Immunogen
This antibody was raised against a fusion protein of amino acids 116-206 of human Mad3.
Application Notes
This antibody is recommended for detection and analysis of Mad3 by Immunocytochemistry and western blot. For instance, the mouse version of this antibody was used to detect Mad3 by western blot in extracts of COS cells transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3, respectively) and untagged Kv2.1 plasmids.
Antibody First Published
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication
Describes the generation of a library of recombinant monoclonal antibodies (R- mAbs) from a pool of mAb-producing hybridomas for neuroscience research.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
