Cat. No.
MABL-206
Application
WB
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
N124B/38R
From
Recombinant Antibody
Specificity
This antibody is specific for Kir2.2. It shows no cross-reactivity against Kir2.1 or Kir2.3. Kir2.2 is an inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues.
Alternative Names
ATP-sensitive inward rectifier potassium channel 12; Kcnj12; Inward rectifier K(+) channel Kir2.2; IRK-2; Potassium channel, inwardly rectifying subfamily J member 12; N124B/38
UniProt
P52188
Immunogen
This antibody was raised against a synthetic peptide of amino acids 390-410 of rat Kir2.2.
Application Notes
FDB and IO muscles of mice were immunoblotted with this antibody (DiFranco et al, 2015; PMID:25545278).
Antibody First Published
DiFranco et al. Inward rectifier potassium currents in mammalian skeletal muscle fibres J Physiol. 2015 Mar 1;593(5):1213-38. PMID:25545278
Note on publication
It was found that Kir2.1 and Kir2.2 channels were targeted to both the surface and the transverse tubular system membrane (TTS) compartments and that both isoforms can be overexpressed up to 3-fold 2 weeks after transfection.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
